Peptide Reconstitution Calculator

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Your inputs

Common protocols

Tap a protocol to auto-fill every field below.

Peptide in vial (mg)

Bacteriostatic water (mL)

Desired dose

Dosing frequency

Used to estimate how long one vial lasts.

Syringe size

All insulin syringes are U-100: 100 units = 1 mL. Barrel size only limits how much fits.

Your results

10 units

draw to this mark · on a 100-unit syringe

050100

Concentration2,500 mcg/mL (2.5 mg/mL)

Volume per dose0.1 mL

Syringe units to draw10 units

Total doses per vial20 doses

Days supply20 days (2w 6d)

DISCLAIMER

This calculator and information are for educational reference only. Always verify your calculations and consult a healthcare provider before using any peptide. Errors in reconstitution can lead to incorrect dosing.

§ COMMON PROTOCOLS

Common reconstitution protocols

Peptide	Vial	BAC water	Typical dose	Volume
Semaglutide	5 mg	2 mL	250 mcg	0.1 mL (10 units)
Tirzepatide	10 mg	2 mL	2.5 mg	0.5 mL (50 units)
Retatrutide	4 mg	2 mL	500 mcg	0.25 mL (25 units)
BPC-157	5 mg	2 mL	250 mcg	0.1 mL (10 units)
TB-500	5 mg	2 mL	2.5 mg	1 mL (100 units)
Ipamorelin	5 mg	2.5 mL	200 mcg	0.1 mL (10 units)
CJC-1295	5 mg	2.5 mL	100 mcg	0.05 mL (5 units)
GHK-Cu	50 mg	5 mL	1.5 mg	0.15 mL (15 units)
Tesamorelin	2 mg	2 mL	2 mg	2 mL (200 units)
DSIP	5 mg	2 mL	150 mcg	0.06 mL (6 units)

Volumes are calculated for a 100-unit (1 mL) insulin syringe. Several of these compounds are research-only and not approved for human use — check each protocol's regulatory status in the peptide library before planning anything.

§ HOW THIS WORKS

How peptide reconstitution works

Peptides come as a lyophilized (freeze-dried) powder in a vial. Before you can inject them, you need to add bacteriostatic water (BAC water) to dissolve the powder. This process is called reconstitution.

The amount of water you add determines the concentration. More water = weaker solution = more volume per dose. Less water = stronger solution = less volume per dose. The total amount of peptide stays the same.

Step-by-step reconstitution guide

Clean the tops of both vials (peptide and BAC water) with alcohol swabs.
Draw the desired amount of BAC water into a syringe.
Insert the needle into the peptide vial at an angle. Let the water run down the side, do not squirt it directly onto the powder.
Gently swirl the vial until the powder fully dissolves. Do not shake.
Store reconstituted peptide in the refrigerator. Use within 30 days.

Tips for accurate reconstitution

Use insulin syringes for drawing doses, more precise than standard syringes for small volumes.
Never shake the vial. Peptides are fragile proteins; shaking can damage them. Swirl gently.
Refrigerate after reconstitution. Most reconstituted peptides are stable for 30 days refrigerated.
Use bacteriostatic water, not sterile water. BAC water contains 0.9% benzyl alcohol as a preservative, allowing multi-dose use.
Double-check your math, or use this calculator. Dosing errors are the most common beginner mistake.

Already reconstituted and only need the syringe mark for a given dose? The dosage calculator does just that step.